Beta-nerve growth factor (beta-NGF), Sheep Polyclonal Antibody

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Catalog Number
S-051

    Product Info

  • Product Name Beta-nerve growth factor (beta-NGF), Sheep Polyclonal Antibody
  • Product Description google Sheep anti-Beta-nerve growth factor (beta NGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
  • Alternative Names Beta-nerve growth factor
  • Application(s) ELISA, IHC-Frozen, Neutralize, WB
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Specificity A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
  • Species Reactivity Avian, Human, Mouse, Rat
  • Immunogen Description Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
  • Conjugate Unconjugated
  • Purity Description Affinity purified antibody
  • Regulatory Status For research use only.

    Specifications

  • Product Description Sheep anti-Beta-nerve growth factor (beta NGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
  • Application(s) ELISA, IHC-Frozen, Neutralize, WB
  • Application Details IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Beta-nerve growth factor (beta NGF)
  • Specificity A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
  • Target Host Species Mouse
  • Species Reactivity Avian, Human, Mouse, Rat
  • Antibody Host Sheep
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
  • Purity Description Affinity purified antibody
  • Format Lyophilized
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Beta-nerve growth factor
  • Uniprot Number P01139
  • Uniprot Number/Name P01139 (NGF_MOUSE)
  • Scientific Background FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Nerve growth factor (NGF) immunoreactivity using sheep polyclonal to native mouse NGF, catalogue number S-051-50.