Casein kinase I isoform alpha (CKI-alpha), Chicken Polyclonal Antibody

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Catalog Number
C-1371

    Product Info

  • Product Name Casein kinase I isoform alpha (CKI-alpha), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Casein kinase I isoform alpha (CKI-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Alternative Names CK1; CKI-alpha;
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Antibody recognizes CK1 by western blot and immunohistochemistry. Cross reactivity
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Immunogen Description Shortest isoform of recombinant full length CK1a.
  • Conjugate Unconjugated
  • Purity Description Concentrated purified IgY
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Casein kinase I isoform alpha (CKI-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB): 1:5,000 - 1:10,000. Casein kinase 1 alpha has a predicted molecular weight of 38 kDa.

    Immunocytochemistry (ICC): 1:500-1:1,000.

    Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Casein kinase I isoform alpha (CKI-alpha)
  • Specificity Antibody recognizes CK1 by western blot and immunohistochemistry. Cross reactivity
  • Target Host Species Human
  • Species Reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Shortest isoform of recombinant full length CK1a.
  • Purity Description Concentrated purified IgY
  • Format Lyophilized IgY preparation, with sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names CK1; CKI-alpha;
  • Uniprot Number P48729
  • Uniprot Number/Name P48729 (KC1A_HUMAN)
  • Scientific Background Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. (Reference: uniprot.org)
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A: Immunofluorescence analysis of casein kinase 1 alpha expression (red) in HeLa cells with C-1371-100. Casein kinase 1 alpha immunoreactivity is observed in the nucleus and the cytoplasm. Nuclear pore complexes (green) were stained with monoclonal antibody M-1397-250. Cell nuclei were visualized with Hoechst dye (blue).
    B: Western blot of whole rat spinal cord homogenate. C-1371-100 (1:10,000 dilution) detects 2 bands at apparent molecular weights of ~38 kDa and ~34 kDa, corresponding to CK1 alpha and related isotypes.

    Citations & References