14-3-3 protein eta, Mouse Monoclonal Antibody

Product Info

Product Name 14-3-3 protein eta, Mouse Monoclonal Antibody
Product Description google Mouse anti-14-3-3 protein eta Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Alternative Names 14.3.3 ; Protein AS1; YWHAH; YWHA1; tyrosine 3-monooxygenase; tryptophan 5-monooxygenase activation protein 1;
Application(s) ICC, IHC-Frozen, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity Human 14-3-3 ETA protein
Species Reactivity Human, Mouse, Rat
Immunogen Description Full length recombinant 14.3.3 protein ETA expressed in and purified from E. coli.
Conjugate Unconjugated
Purity Description Protein G purified
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-14-3-3 protein eta Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Application(s) ICC, IHC-Frozen, WB
Application Details WB, ICC, IHC. Suggested dilution of 1:500-1:1,000 for IHC and ICC. Suggested dilution of 1:1,000-1:5,000 for WB. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Target 14-3-3 protein eta
Specificity Human 14-3-3 ETA protein
Target Host Species Human
Species Reactivity Human, Mouse, Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG
Clone Name 3G12
Conjugate Unconjugated
Immunogen Description Full length recombinant 14.3.3 protein ETA expressed in and purified from E. coli.
Purity Description Protein G purified
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names 14.3.3 ; Protein AS1; YWHAH; YWHA1; tyrosine 3-monooxygenase; tryptophan 5-monooxygenase activation protein 1;
Uniprot Number Q04917
Uniprot Number/Name Q04917 (1433F_HUMAN)
Scientific Background 14.3.3 protein eta or 14.3.3 binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (Ref SwissProt). 14.3.3 protein eta is widely expressed as both homodimers and heterodimers and are concentrated in the nervous system. High concentrations of 14.3.3 protein eta have been linked to Creutzfeld Jacob Disease, Parkinson's Disease and early-onset schizopherenia.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Image shows rat mixed neuron/glial cultures stained with Mouse monoclonal antibody to 14.3.3 protein eta M-1572-100 antibody (red). Neuronal perikarya are very rich in 14.3.3 protein eta which has a diffuse cytoplasmic staining pattern. Blue is a DNA stain.
Left: Analysis of 14.3.3 eta expression in HeLa cells by Immuncytochemistry using mouse antibody to 14.3.3 protein eta (red, 1:1,000). Blue: DAPI nuclear stain. The antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region. Right: Western blot analysis of whole brain lysates (lanes 2,3), and cell lysates (lanes 4-8), using mouse antibody to 14.3.3 eta (green, 1:5,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells. The strong band at 28 kDa corresponds to 14.3.3 eta protein.

Citations & References