Cytokeratin 18 (CK-18), Mouse Monoclonal Antibody

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Catalog Number
M-1168

    Product Info

  • Product Name Cytokeratin 18 (CK-18), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Cytokeratin 18 (CK-18) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Alternative Names Keratin; type I cytoskeletal 18; Cytokeratin-18; CK-18; Keratin-18; K18; Cell proliferation-inducing gene 46 protein; KRT18; CYK18; PIG46;
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
  • Species Reactivity Human, Rat
  • Immunogen Description Human epidermal carcinoma A431 and MCF7 human breast cancer cell lines
  • Conjugate Unconjugated
  • Purity Description IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Cytokeratin 18 (CK-18) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
  • Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
  • Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human Cytokeratin 18 has a predicted length of 430 amino acids and MW of 48 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. A concentration of 0.5-2.0 µg/mL is recommended to detect the protein in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Cytokeratin 18 (CK-18)
  • Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
  • Target Host Species Human
  • Species Reactivity Human, Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name CK-18
  • Conjugate Unconjugated
  • Immunogen Description Human epidermal carcinoma A431 and MCF7 human breast cancer cell lines
  • Purity Description IgG
  • Format Liquid (0.5 mL). 50% glycerol, 0.9 mg NaCl and 0.2 mg Na2HPO4
  • Reconstitution Instructions The liquid formulation should be diluted in PBS (pH 7.4)
  • Storage Instructions After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Keratin; type I cytoskeletal 18; Cytokeratin-18; CK-18; Keratin-18; K18; Cell proliferation-inducing gene 46 protein; KRT18; CYK18; PIG46;
  • Uniprot Number P05783
  • Uniprot Number/Name P05783 (K1C18_HUMAN)
  • Scientific Background Cytokeratin 18 is an acidic type I keratin and a member of intermediate filament family of proteins. Cytokeratin 18 typically forms a heterotetramer with Cytokeratin 8 to form an intermediate filament in simple single-layered epithelial cells. Cytokeratin 18 is expressed primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

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