Gremlin-1, Mouse Monoclonal Antibody

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Catalog Number
M-877

    Product Info

  • Product Name Gremlin-1, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Gremlin-1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Cysteine knot superfamily 1 BMP antagonist 1; Increased in high glucose protein 2; IHG-2; Down-regulated in Mos-transformed cells protein; DAN domain family member 2; Cell proliferation-inducing gene 2 protein GREM1; CKTSF1B1; DAND2; DRM
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
  • Species Reactivity Human
  • Immunogen Description Partial recombinant human GremLin-1 (75-184) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Gremlin-1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Gremlin-1
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG2a, kappa
  • Clone Name 4C2
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human GremLin-1 (75-184) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.4
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions lyophilized antibody can be stored at -20°C. After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Cysteine knot superfamily 1 BMP antagonist 1; Increased in high glucose protein 2; IHG-2; Down-regulated in Mos-transformed cells protein; DAN domain family member 2; Cell proliferation-inducing gene 2 protein GREM1; CKTSF1B1; DAND2; DRM
  • Uniprot Number O60565
  • Uniprot Number/Name O60565 (GREM1_HUMAN)
  • Scientific Background
    GremLin-1 is a secreted cytokine that may play a role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. It is highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. GremLin-1 is induced through TGF-beta 1-mediated pathways in Mesangial cell and it is down-regulated in tumor cell lines.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of GST tagged recombinant human Gremlin-1. Note the GST tag alone is 26 kDa.

  • Detection limit of 3 ng/mL for GST tagged recombinant Gremlin-1 when the anti-Gremlin1 primary antibody, catalogue number M-877-100, is used as a capture antibody in Sandwich ELISA.

    Citations & References