Product DescriptiongoogleMouse anti-Gremlin-1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Alternative NamesCysteine knot superfamily 1 BMP antagonist 1; Increased in high glucose protein 2; IHG-2; Down-regulated in Mos-transformed cells protein; DAN domain family member 2; Cell proliferation-inducing gene 2 protein GREM1; CKTSF1B1; DAND2; DRM
Application(s)ELISA, WB
Antibody HostMouse
Antibody TypeMonoclonal
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen.
Species ReactivityHuman
Immunogen DescriptionPartial recombinant human GremLin-1 (75-184) with a GST tag.
ConjugateUnconjugated
Purity DescriptionProtein G purified immunoglobulin
Product DescriptionMouse anti-Gremlin-1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGremlin-1
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG2a, kappa
Clone Name4C2
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human GremLin-1 (75-184) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.4
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructionslyophilized antibody can be stored at -20°C. After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCysteine knot superfamily 1 BMP antagonist 1; Increased in high glucose protein 2; IHG-2; Down-regulated in Mos-transformed cells protein; DAN domain family member 2; Cell proliferation-inducing gene 2 protein GREM1; CKTSF1B1; DAND2; DRM
GremLin-1 is a secreted cytokine that may play a role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. It is highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. GremLin-1 is induced through TGF-beta 1-mediated pathways in Mesangial cell and it is down-regulated in tumor cell lines.
Western blot detection of GST tagged recombinant human Gremlin-1. Note the GST tag alone is 26 kDa.
Detection limit of 3 ng/mL for GST tagged recombinant Gremlin-1 when the anti-Gremlin1 primary antibody, catalogue number M-877-100, is used as a capture antibody in Sandwich ELISA.