Hairy-related transcription factor 3 (hHRT3), Mouse Monoclonal Antibody

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Catalog Number
M-894

    Product Info

  • Product Name Hairy-related transcription factor 3 (hHRT3), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Hairy-related transcription factor 3 (hHRT3) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names Hairy/enhancer-of-split related with YRPW motif-like protein; Hairy-related transcription factor 3; hHRT3; hHeyL; HEYL; HRT3
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human and mouse. Other species have not been tested.
  • Species Reactivity Human, Mouse
  • Immunogen Description Partial recombinant human hHRT3 (221-326) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Hairy-related transcription factor 3 (hHRT3) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB, immunofluorescence and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Hairy-related transcription factor 3 (hHRT3)
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human and mouse. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human, Mouse
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 4A11
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human hHRT3 (221-326) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Hairy/enhancer-of-split related with YRPW motif-like protein; Hairy-related transcription factor 3; hHRT3; hHeyL; HEYL; HRT3
  • Uniprot Number Q9NQ87
  • Uniprot Number/Name Q9NQ87 (HEYL_HUMAN)
  • Scientific Background
    hHRT3 belongs to HESR family of basic helix-loop-helix type transcription factors. It contains a bHLH and orange domain, but its YPRW motif has diverged from other HESR family members. It is believed to be a downstream effector of Notch signaling which may be required for cardiovascular development.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunofluorescent detection of hHRT3 expression in HeLa cells. The anti-hHRT3 primary antibody, catalogue number M-894-100, was used at a concentration of 30 µg/mL.

  • Western blot detection of hHRT3 expression in HeLa S3 nuclear exctract lysate.

    Citations & References