Application DetailsThis antibody is recommended for WB, immunofluorescence and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human and mouse. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name4A11
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human hHRT3 (221-326) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHairy/enhancer-of-split related with YRPW motif-like protein; Hairy-related transcription factor 3; hHRT3; hHeyL; HEYL; HRT3
hHRT3 belongs to HESR family of basic helix-loop-helix type transcription factors. It contains a bHLH and orange domain, but its YPRW motif has diverged from other HESR family members. It is believed to be a downstream effector of Notch signaling which may be required for cardiovascular development.
Immunofluorescent detection of hHRT3 expression in HeLa cells. The anti-hHRT3 primary antibody, catalogue number M-894-100, was used at a concentration of 30 µg/mL.
Western blot detection of hHRT3 expression in HeLa S3 nuclear exctract lysate.