Product DescriptionMouse anti-Homeobox protein DLX-5 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for direct and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHomeobox protein DLX-5
SpecificityThe specificity of this antibody was confirmed by direct ELISA against the antigen. No other species have been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name1B3
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant protein of human homeobox protein DLX-5 (amino acids 1 to 110) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Homeobox protein DLX-5 belongs to the the distal-less homeobox family. It contains one homeobox DNA-binding domain and is thought to play a role in bone development and fracture healing. It is first expressed at 8.5 to 9 days in facial and branchial arch mesenchyme, otic vesicles and frontonasal ectoderm around olfactory placodes. At day 9.5-10, expression is seen in the developing forebrain. In day 12.5 embryos it is expressed in the brain and bones and also in all skeletal structures of mid gestation embryos after the first cartilage formation. Expression remains unaltered in both brain and skeleton in day 15 embryos and slowly decreases in day 17 embryos. The unprocessed precursor has a length of 289 amino acids and a predicted molecular weight of 31.5 kDa.
Sandwich ELISA using mouse monoclonal antibody to human homeobox protein DLX-5 [1B3], catalogue number M-825-100, as a capture antibody. The detection limit for recombinant GST tagged homeobox protein DLX-5 is 0.3ng/ml.