Product DescriptionMouse anti-Segment polarity protein dishevelled homolog DVL-3 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSegment polarity protein dishevelled homolog DVL-3
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name4H2
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant protein of human DSH homolog 3 (aa 1 to 99) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSegment polarity protein dishevelled homolg DVL-3; Dishevellled-3; DVL3
DSH-homolog 3 may play a role in the signal transduction pathway mediated by multiple Wnt genes. It is a cytoplasmic protein that belongs to the DSH family. It contains a DEP domain, a DIX domain and a PDZ (DHR) domain. It interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2. The unprocessed precursor has a length of 716 amino acids and an estimated molecular weight of 78 kDa.
Western blot analysis on human epidermoid carcinoma lysate using mouse monoclonal antibody to DSH homology 3, catalogue number M-828-50.
Sandwhich ELISA using mouse monoclonal antibody to DSH homolog 3, catalogue number M-828-50, as a capture antibody. The detection limit for recombinant human GST tagged DSH homolog 3 is 0.3 ng/mL.
Immunofluorescence of mouse monoclonal antibody to human DSH homolog 3, at a concentration of 10 µg/mL, on human epidermoid carcinoma cell lysate.