Application DetailsThis antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetOrphan nuclear receptor TR4
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name2A5
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human Orphan nuclear receptor TR4 (amino acids 43-153) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNuclear receptor subfamily 2 group C member 2; Orphan nuclear receptor TAK1; Testicular receptor 4; NR2C2; TAK1; TR4
Members of the nuclear hormone receptor family, such as Orphan nuclear receptor TR4, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. Orphan nuclear receptor TR4 may regulate gene expression during the late phase of spermatogenesis. It is transiently repressed during the meiotic phase of spermatogenesis.
Western blot detection of human Orphan nuclear receptor TR4 expression in transfected 293T cell lysate. Lane 1: transfected 293T cell lysate. Lane 2: Non-transfected 293T cell lysate.