Peripherin, Mouse Monoclonal Antibody

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Catalog Number
M-1400

    Product Info

  • Product Name Peripherin, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Peripherin Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Alternative Names Peripherin; Prph; Prph1;
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. Human, mouse, feline. Predicted to react with other mammalian tissue.
  • Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
  • Immunogen Description Recombinant full length rat Peripherin protein expressed in and purified from E.coli
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Peripherin Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A concentration of 0.5 - 2 µg/mL is recommended for WB. A concentration of 1-5 µg/mL is recommended for IC and IH. This antibody performs well on aldehyde fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Peripherin
  • Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. Human, mouse, feline. Predicted to react with other mammalian tissue.
  • Target Host Species Rat
  • Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 7C5
  • Conjugate Unconjugated
  • Immunogen Description Recombinant full length rat Peripherin protein expressed in and purified from E.coli
  • Purity Description Protein G purified
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Peripherin; Prph; Prph1;
  • Uniprot Number P21807
  • Uniprot Number/Name P21807 (PERI_RAT)
  • Scientific Background Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Mixed neuron/glia cultures from newborn rat brain stained with Mouse monoclonal antibody to Peripherin [7C5] (green) and Rabbit polyclonal antibody to NF-L R-1392-50 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not Peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both Peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.

    Citations & References

  • Specific References Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.