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Sodium Potassium ATPase - alpha subunit, Mouse Monoclonal Antibody
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Catalog Number
M-985
Discontinued
- Product Name Sodium Potassium ATPase - alpha subunit, Mouse Monoclonal Antibody
- Product Description Mouse anti-Sodium Potassium ATPase - alpha subunit Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Na;K-ATPase; ATP1A1; Atpa-1;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; chicken;
- Species Reactivity Chicken, Human, Sheep
- Immunogen Description Sheep kidney alpha subunit of Sodium Potassium ATPase. The epitope recognized by this antibody is between amino acids 646 and 652.
- Conjugate Unconjugated
- Purity Description IgG
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-Sodium Potassium ATPase - alpha subunit Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Boiling the protein is not recommended as it causes aggregation. Human Na+/K+ ATPase subunit alpha 1 has a predicted length of 1,023 amino acids and a MW of 113 kDa. A concentration of 4.0-8.0 µg/mL is recommended to detect Na+/K+ ATPase subunit alpha 1 in formalin or acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Sodium Potassium ATPase - alpha subunit
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; chicken;
- Target Host Species Sheep
- Species Reactivity Chicken, Human, Sheep
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG
- Clone Name IMD-66
- Conjugate Unconjugated
- Immunogen Description Sheep kidney alpha subunit of Sodium Potassium ATPase. The epitope recognized by this antibody is between amino acids 646 and 652.
- Purity Description IgG
- Format Lyophilized from 1.2% sodium acetate, 2 mg BSA, 0.01 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Na;K-ATPase; ATP1A1; Atpa-1;
- Uniprot Number P05023
- Uniprot Number/Name P05023 (AT1A1_HUMAN)
- Scientific Background Na+/K+ ATPase is an integral membrane heterodimer belonging to the family of P-type cation transport ATPases. The enzyme is comprised of a large catalytic alpha subunit and a smaller beta subunit. The alpha subunit catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.