E1A binding protein p300, Mouse Monoclonal Antibody

Product Info

Product Name E1A binding protein p300, Mouse Monoclonal Antibody
Product Description google Mouse anti-E1A binding protein p300 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Alternative Names EP300
Application(s) ELISA, IHC-Frozen, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Species Reactivity Human
Immunogen Description Partial recombinant protein of human E1A binding protein (aa 731 to 830) with a GST tag.
Conjugate Unconjugated
Purity Description Protein G purified immunoglobulin
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-E1A binding protein p300 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Application(s) ELISA, IHC-Frozen, WB
Application Details This antibody is recommended for WB, IHC, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target E1A binding protein p300
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host Species Human
Species Reactivity Human
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG
Clone Name 1D2
Conjugate Unconjugated
Immunogen Description Partial recombinant protein of human E1A binding protein (aa 731 to 830) with a GST tag.
Purity Description Protein G purified immunoglobulin
Format Lyophilized from PBS pH 7.2
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names EP300
Uniprot Number B1AKC2
Uniprot Number/Name Q09472 (EP300_HUMAN)
Scientific Background
E1A binding protein p300 is an E1A-associated cellular p300 transcriptional co-activator protein. It is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. E1A binding protein p300 activity is specifically inhibited by the adenovirus oncoprotein E1A. It has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Immunohistochemical detection of E1A binding protein p300 in formalin-fixed and paraffin-embedded human colon tissue using mouse monoclonal to human E1A binding protein p300, catalogue number M-833-50, at a concentration of 3 µg/mL.
Immunofluorescence of mouse monoclonal antibody to human E1A binding protein p300, catalogue number M-833-50, at a concentration of 10 µg/mL, on HeLa cell lysate.

Western blot detection of E1A binding protein p300 in HeLa S3 nuclear extract lysate using mouse monoclonal to human E1A binding protein p300, catalogue number M-833-50.

Citations & References