SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
Species ReactivityHuman, Mouse, Rabbit, Rat
Immunogen DescriptionA synthetic peptide (EYEKEKLNERLAKLSDGV) corresponding to a region (384-401) from human Heat Shock Protein 60 (HSP60). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human HSP60 has a predicted length of 573 residues and MW of 61 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHeat Shock Protein 60 (HSP-60)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; rabbit;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rabbit, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (EYEKEKLNERLAKLSDGV) corresponding to a region (384-401) from human Heat Shock Protein 60 (HSP60). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Scientific BackgroundHeat Shock Protein 60 (HSP60) is a chaperonin with roles in mitochondrial protein import and folding of polypeptides in the mitochondrial matrix.
Immunohistochemical staining of paraffin-embedded human intestinal cancer sections using Heat Shock Protein 60 (R-1065-100) at a concentration of 0.5-1.0 µg/ml. Secondary detection using Biotinylated goat anti-rabbit IgG (10 µg/ml).