SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide (WYEGVCNRVTGL) corresponding to a region (483-494) from human Abelson interactor 1 (ABI1). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
ConjugateUnconjugated
Purity DescriptionAffinity purified on antigen column
Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human ABI1 (isoform 1) has a predicted length of 508 residues and MW of 55 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect ABI1 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetAbelson interactor 1 (Abi-1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (WYEGVCNRVTGL) corresponding to a region (483-494) from human Abelson interactor 1 (ABI1). To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity DescriptionAffinity purified on antigen column
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAbelson interactor; Abi-1; Spectrin SH3 domain-binding protein 1; Eps8 SH3 domain-binding protein; Eps8-binding protein; e3B1; Nap1-binding protein; Nap1BP; Abl-binding protein 4; AblBP4; SSH3BP1;
Scientific BackgroundAbelson interactor 1 (ABI1) is widely expressed with highest expression in brain. ABI1 in participation with EPS8 and SOS1 is involved in the transduction of signals from Ras to Rac. At least 10 isoforms are produced by alternative splicing.