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Lamin-B1, Rabbit Polyclonal Antibody
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Catalog Number
R-1088
- Product Name Lamin-B1, Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Lamin-B1 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names LMNB1; LMN2; LMNB;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Species Reactivity Human, Mouse, Rat
- Immunogen Description A synthetic peptide (FHQQGTPRASNRSCAIM) corresponding to amino acids 570-586 from human Lamin B1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Lamin-B1 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Lamin B1 (precursor) has a predicted length of 586 residues and MW of 66 kDa. A concentration of 1.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0 µg/mL is also recommended for formalin/acetone fixed tissues. Antigen retrieval by Pepsin and Trypsin is required in acetone fixed cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Lamin-B1
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
- Target Host Species Human
- Species Reactivity Human, Mouse, Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (FHQQGTPRASNRSCAIM) corresponding to amino acids 570-586 from human Lamin B1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names LMNB1; LMN2; LMNB;
- Uniprot Number P20700
- Uniprot Number/Name P20700 (LMNB1_HUMAN)
- Scientific Background Lamins belong to the intermediate filament family of proteins. They are structural components of the nuclear lamina and are thought to be involved in nuclear stability, chromatin structure and gene expression. There are two main types of lamins; A and B. The A type lamins include A, C and Adel10. The B-type lamins, B1 and B2, arise from two distinct genes.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.