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Protein kinase C alpha (PKC-A), Rabbit Polyclonal Antibody
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Catalog Number
R-1748
- Product Name Protein kinase C alpha (PKC-A), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Protein kinase C alpha (PKC-A) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names PKC-alpha; PKC-A; EC 2.7.11.13; PRKCA; PKCA; PRKACA;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; predicted to react with mouse and rat due to sequence similarity.
- Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
- Immunogen Description A synthetic peptide corresponding to a sequence of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), that differs from the related rat and mouse sequences by one amino acid. There is 100% alignment with various monkey species including Rhesus, Baboon, gorillas by BLAST as well as other mammals including horse and marmoset.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Protein kinase C alpha (PKC-A) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Protein kinase C alpha has a predicted length of 672 residues and MW of 77 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in paraffin embedded tissues. Antigen retrieval by boiling paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Protein kinase C alpha (PKC-A)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; predicted to react with mouse and rat due to sequence similarity.
- Target Host Species Human
- Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide corresponding to a sequence of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), that differs from the related rat and mouse sequences by one amino acid. There is 100% alignment with various monkey species including Rhesus, Baboon, gorillas by BLAST as well as other mammals including horse and marmoset.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute vial in 0.2 mL sterile-filtered, ultrapure water. This will produce a final concentration of 500 µg/mL of antibody. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at -20°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to 6 months for higher stability.Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names PKC-alpha; PKC-A; EC 2.7.11.13; PRKCA; PKCA; PRKACA;
- Uniprot Number P17252
- Uniprot Number/Name P17252 (KPCA_HUMAN)
- Scientific Background Protein kinase C alpha (PKC-alpha) belongs to the Protein kinase C family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC-alpha is one of at least 12 different types of Protein kinase C identified in humans. PKC-alpha is ubiquitously expressed and has roles in many different cellular processes including cell adhesion, cell transformation, proliferation, differentiation and cell cycle checkpoint.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
