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Smad 2/3, Rabbit Polyclonal Antibody
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Catalog Number
R-1002
Discontinued
- Product Name Smad 2/3, Rabbit Polyclonal Antibody
- Product Description Rabbit anti-SMAD2/3 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names Mad3; SMAD 3; Mothers against DPP homolog 3; Mothers against DPP homolog 2; MAD-2; SMAD 2; JV18-1; MADH2; MADH3;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; expected to react with rat and mouse due to sequence homology
- Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
- Immunogen Description A synthetic peptide (EQNGQEEKWCEKAVKS) corresponding to a region from the N-terminus of human Smad2 and Smad3.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-SMAD2/3 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The predicted length of human Smad2 is 467 amino acids and MW of 52 kDa. The predicted length of Smad3 is 425 amino acids and MW of 48 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Smad 2/3 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Heat mediated antigen retrieval is required. A concentration of 0.4-1.0 µg/mL is recommended for IHC-F. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target SMAD2/3
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; expected to react with rat and mouse due to sequence homology
- Target Host Species Human
- Species Reactivity Human, Mouse (Predicted), Rat (Predicted)
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide (EQNGQEEKWCEKAVKS) corresponding to a region from the N-terminus of human Smad2 and Smad3.
- Purity Description Affinity purified on antigen column
- Format Liquid (0.5 mL). 50% glycerol, 0.9 mg NaCl and 0.2 mg Na2HPO4
- Storage Instructions Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Mad3; SMAD 3; Mothers against DPP homolog 3; Mothers against DPP homolog 2; MAD-2; SMAD 2; JV18-1; MADH2; MADH3;
- Uniprot Number Q15796; P84022
-
Uniprot Number/Name
Q15796 SMAD2_HUMAN)
P84022 (SMAD3_HUMAN) - Scientific Background Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription. Smad4, also known as the common-mediator Smad (co-Smad), interacts with the receptor-regulated Smad proteins (R-SMAD). The R-SMAD class includes Smad2 and Smad3.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.