Signal transducer and activator of transcription 1-alpha (STAT1 alpha), Rabbit Polyclonal Antibody

Product Info

Product Name Signal transducer and activator of transcription 1-alpha (STAT1 alpha), Rabbit Polyclonal Antibody
Product Description google
Rabbit anti-Signal transducer and activator of transcription 1-alpha (STAT1 alpha) Polyclonal Antibody (Unconjugated), suitable for WB.
Alternative Names
Transcription factor ISGF-3 components p91/p84; STAT1; p91.
Application(s) WB
Antibody Host Rabbit
Antibody Type Polyclonal
Specificity
The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; rabbit.
Species Reactivity Human, Mouse, Rabbit, Rat
Immunogen Description A synthetic peptide of human STAT1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Conjugate Unconjugated
Purity Description Affinity purified on antigen column
Regulatory Status For research use only.

Specifications

Product Description
Rabbit anti-Signal transducer and activator of transcription 1-alpha (STAT1 alpha) Polyclonal Antibody (Unconjugated), suitable for WB.
Application(s) WB
Application Details Western Blotting (WB). A concentration of 1.0-2.0 μg/mL is recommended for WB. Human STAT1 (isoform alpha/p91) has a predicted length of 750 residues and MW of 87 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Signal transducer and activator of transcription 1-alpha (STAT1 alpha)
Specificity
The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; rabbit.
Target Host Species Human
Species Reactivity Human, Mouse, Rabbit, Rat
Antibody Host Rabbit
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide of human STAT1. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Purity Description Affinity purified on antigen column
Format Lyophilized from a solution containing 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 200 μL sterile-filtered, ultrapure water to achieve a 0.5 mg/mL concentration. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability (up to 6 months). Avoid freeze-thaw cycles. Reconstituted antibody can be stored for 1 month at 2-8°C. Unopened, lyophilized vial may be stored for 12 months from date of receipt at -20°C.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names
Transcription factor ISGF-3 components p91/p84; STAT1; p91.
Uniprot Number P42224
Uniprot Number/Name P42224 (STAT1_HUMAN)
Scientific Background STAT1 belongs to the STAT (signal transducers and activators of transcription) family of proteins. STAT proteins undergo tyrosine/serine phosphorylation in response to cytokines and growth factors. As a result of this phosphorylation, STAT proteins form dimers and are translocated to the nucleus where they act as transcription factors. Alternative splicing of the STAT1 gene gives rise to the STAT1 alpha and beta isoforms.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Analysis by western blot of STAT-1 expression in mouse tissue lysates with strong bands at approx 87-91kDa. Lane 1: heart; Lane 2: liver; Lane 3: brain; Lane 4: kidney; Lane 5: spleen; Lane 6: thymus; Lane 7: lung; Lane 8: intestine; Lane 9: ovary. Lower molecular weight bands that result from degradation during processing can also be detected; some can be quite prominent if insufficient protease inhibitors are used, particularly in susceptible tissues such as the kidney and spleen.

Method: For 2-3 hours, electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel). Each lane's sample well was loaded with 50ug of sample under reducing conditions. The membrane was then incubated with rabbit anti-STAT1 (R-1103-100) at 0.5 g/mL overnight at 4°C, then washed three times with TBS-0.1%Tween for five minutes each before being probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000 for 1.5 hour at room temperature. An Enhanced Chemiluminescent Detection is used to generate the signal.

Citations & References