Mothers against decapentaplegic homolog 1,2,3,5 (Smad 1,2,3,5), Rabbit Polyclonal Antibody

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Catalog Number
R-1368
Discontinued

    Product Info

  • Product Name Mothers against decapentaplegic homolog 1,2,3,5 (Smad 1,2,3,5), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Mothers against decapentaplegic homolog 1,2,3,5 (Smad 1,2,3,5) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Alternative Names Mothers against decapentaplegic homolog; MAD homolog; Mothers against DPP homolog; JV4-1; Mad-related protein;SMAD family member; SMAD 1; SMAD 2; SMAD 3; SMAD 5; Smad1; Smad2; Smad3; Smad5; hSMAD1; hSMAD2; hSMAD3; hSMAD5; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; SMAD2; SMAD3; SMAD5; BSP1; MADH1; MADR1; JV18-1; hMAD-2; Mad3; hMAD-3; hMAD-2; MADH2; MADH3; MADH5; JV5-1;
  • Application(s) WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human and rat; predicted to react with mouse due to sequence homology;
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Immunogen Description A synthetic peptide (GPLQWLDKVLTQMGS) corresponding to a region (442-456 aa) from the C-terminus of human Smad 1,2,3,5.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Mothers against decapentaplegic homolog 1,2,3,5 (Smad 1,2,3,5) Polyclonal Antibody (Unconjugated), suitable for WB.
  • Application(s) WB
  • Application Details Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Mothers against decapentaplegic homolog 1,2,3,5 (Smad 1,2,3,5)
  • Specificity The specificity of this antibody has been confirmed by WB against the antigen. Human and rat; predicted to react with mouse due to sequence homology;
  • Target Host Species Human
  • Species Reactivity Human, Mouse (Predicted), Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (GPLQWLDKVLTQMGS) corresponding to a region (442-456 aa) from the C-terminus of human Smad 1,2,3,5.
  • Sequence GPLQWLDKVLTQMGS
  • Purity Description Affinity purified
  • Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Mothers against decapentaplegic homolog; MAD homolog; Mothers against DPP homolog; JV4-1; Mad-related protein;SMAD family member; SMAD 1; SMAD 2; SMAD 3; SMAD 5; Smad1; Smad2; Smad3; Smad5; hSMAD1; hSMAD2; hSMAD3; hSMAD5; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; SMAD2; SMAD3; SMAD5; BSP1; MADH1; MADR1; JV18-1; hMAD-2; Mad3; hMAD-3; hMAD-2; MADH2; MADH3; MADH5; JV5-1;
  • Uniprot Number Q15797; Q15796; P84022; Q99717
  • Uniprot Number/Name Q15797 (SMAD1_HUMAN)
    Q15796 (SMAD2_HUMAN)
    P84022 (SMAD3_HUMAN)
    Q99717 (SMAD5_HUMAN)
  • Scientific Background Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot using Rabbit polyclonal antibody to Smad 1,2,3,5:
    Lane 1 : Rat heart tissue lysate
    Lane 2: Rat skeletal muscle tissue lysate
    Lane 3: Rat kidney tissue lysate
    Lane 4: Rat brain tissue lysate
    Lane 5: MM453 Whole cell lysate
    Lane 6: MM231 Whole cell lysate
    Lane 7: HeLa Whole cell lysate
    Lane 8: SMMC Whole cell lysate
    Lane 9: SW620 Whole cell lysate

    Citations & References