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Cellular oncogene fos (c-Fos), Sheep Polyclonal Antibody

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Catalog Number

S-033

Product Info

Product Name Cellular oncogene fos (c-Fos), Sheep Polyclonal Antibody
Product Description google Sheep anti-Cellular oncogene fos (c-Fos) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names c-Fos; Proto-oncogene protein cFOS; cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7
Application(s) IHC-Frozen, WB
Antibody Host Sheep
Antibody Type Polyclonal
Specificity This antiserum shows a high level of specificity for c-FOS confirmed by immunohistochemistry. This antiserum is known to react with rat and rabbit and hamster cFOS.
Species Reactivity Hamster, Human, Rabbit, Rat
Immunogen Description A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Conjugate Unconjugated
Purity Description Protein G purified.
Regulatory Status For research use only.

Specifications

Product Description Sheep anti-Cellular oncogene fos (c-Fos) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details IHC: Use at an amount of 10 µg/mL with an incubation time of 1-3 days at 4ºC. This antiserum works in paraffin and 4% PFA fixed frozen sections. Penetration is the key to success. Over-fixed tissue is problematic. Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Cellular oncogene fos (c-Fos)
Specificity This antiserum shows a high level of specificity for c-FOS confirmed by immunohistochemistry. This antiserum is known to react with rat and rabbit and hamster cFOS.
Target Host Species Human
Species Reactivity Hamster, Human, Rabbit, Rat
Antibody Host Sheep
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Purity Description Protein G purified.
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names c-Fos; Proto-oncogene protein cFOS; cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7
Uniprot Number P01100
Uniprot Number/Name P01100 (FOS_HUMAN)
Scientific Background FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

cFos was induced in the hypothalamic arcuate nucleus of an adult male Wistar rat using i.p. injection of N-methyl-D-aspartate. The brain was fixed by perfusion with formalin (4%). cFos immunoreactivity was detected in floated cryo-sections of the hypothalamic arcuate nucleus with the S-033-500 primary antibody (1:30 000) using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Citations & References

General References OEM supplier to Chemicon/EMD Millipore/MilliporeSigma as Chemicon(R) AB1584 Sheep anti-c-Fos antibody since 1999. Check representative websites for publication references Vanstraaten et al (1983) Proc. Natl. Acad. Sci. 80: 318Original molecular sequence paper
Minson J. et al. (1994) Brain Res. 646: 44-5Early immunolocalization paper