Product DescriptionMouse anti-Neuropilin-1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeuropilin-1
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG2a, kappa
Clone Name1B3
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant human Neuropilin-1 (22-131) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.4
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
The antibody is made against the membrane-bound isoform 1 of Neuropilin-1. It is a receptor involved in the development of the cardiovascular systerm, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It binds both vascular endothelial growth factor (VEGF) and semaphorin family members. Isoform 1 is expressed by blood vessels of different tissues. In the developing embryo it is predominantly found in the nervous system. In adult tissues, it is highly expressed in heart and placenta, moderately expressed in lung, liver and skeletal muscle, kidney and pancreas, and low in adult brain.
Western blot detection of GST tagged recombinant human Neuropilin-1. Note the GST tag alone is 26 kDa.
Detection limit of 0.03 ng/mL for GST tagged recombinant Neuropilin-1 when the anti-Neuropilin-1 primary antibody, catalogue number M-878-100 is used as a capture antibody in sandwich ELISA.