NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (ATTO 488)

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Catalog Number
M-027-AT

    Product Info

  • Product Name NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (ATTO 488)
  • Product Description google Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (ATTO 488), suitable for ICC.
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Application(s) ICC
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
  • Species Reactivity Cat, Dog, Human, Pig, Rabbit, Sheep
  • Immunogen Description The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
  • Conjugate ATTO 488
  • Purity Description Protein G purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (ATTO 488), suitable for ICC.
  • Application(s) ICC
  • Application Details This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 ¬µg/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 ¬µg/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target NGFR/p75 neurotrophin receptor (p75NTR)
  • Specificity This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
  • Target Host Species Human
  • Species Reactivity Cat, Dog, Human, Pig, Rabbit, Sheep
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1, kappa
  • Clone Name ME20.4
  • Conjugate ATTO 488
  • Ex/Em Max Excitation maximum: 500 nm. Emission maximum: 520 nm.
  • Immunogen Description The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
  • Purity Description Protein G purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
  • Format Liquid (1 mg/mL) in PBS, pH 7.2-7.6 without preservatives. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
  • Storage Instructions Aliquot antibody and store frozen at -20°C to -80°C. For short-term storage, the antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent, or for up to 1 week without the addition of a preservative.
  • Batch Number Please see item label.
  • Expiration Date 4 months after date of receipt (unopened vial)
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Uniprot Number P08138
  • Uniprot Number/Name P08138 (TNR16_HUMAN)
  • Scientific Background Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.