Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 3.5 µg/mL is recommended for WB. The predicted length of human actinin (isoform #1) is 892 residues and a MW of 103 kDa. A concentration of 3.5-7.0 µg/mL is recommended to detect alpha actinin in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Also the recommended titre for formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetActinin, skeletal muscle alpha
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundActinin is a F-actin cross linking protein and is thought to anchor actin to a variety of intracellular structures. At least 4 isoforms of alpha actinin have been found. This antibody reacts specifically with alpha actinin isoform #1.