Left: SH-SY5Y neuroblastoma cells stained with chicken antibody to Nestin (green, 1:2,000) and rabbit antibody to Vimentin (R-1699-100, red, 1:2,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in rat C6 (Lane 1) and human SH-SY5Y (Lane 2) cell lysates. The antibody (1:1,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.