Beta-nerve growth factor (Beta-NGF), Rabbit Polyclonal Antibody

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Catalog Number
R-093

    Product Info

  • Product Name Beta-nerve growth factor (Beta-NGF), Rabbit Polyclonal Antibody
  • Product Description google
    Rabbit anti-Beta-nerve growth factor (Beta-NGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
  • Alternative Names Beta-nerve growth factor
  • Application(s) ELISA, IHC-Frozen, Neutralize, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
  • Species Reactivity Avian, Human, Mouse, Rat
  • Immunogen Description Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
  • Conjugate Unconjugated
  • Purity Description Protein G purified IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Rabbit anti-Beta-nerve growth factor (Beta-NGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
  • Related Products Nerve growth factor (2.5S), Mouse, Purified Native Protein
    Beta-nerve growth factor (beta-NGF), Rabbit Polyclonal Antibody
  • Application(s) ELISA, IHC-Frozen, Neutralize, WB
  • Application Details IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Beta-nerve growth factor (Beta-NGF)
  • Specificity A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
  • Target Host Species Mouse
  • Species Reactivity Avian, Human, Mouse, Rat
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
  • Purity Description Protein G purified IgG
  • Format Lyophilized from PBS, pH 7.2-7.6 without preservatives
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Beta-nerve growth factor
  • Uniprot Number P01139
  • Uniprot Number/Name P01139 (NGF_MOUSE)
  • Scientific Background FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot analysis of NGF expression in rodent and human homogenates. Mature NGF is detected in mouse salivary gland homogenate at ~13 kDa, while tissue samples reveal predominantly proNGF isoforms at higher molecular weights. This is consistent with the observation that proNGF is the predominant isoform in brain tissue (Fahnenstock et al., 2001). Several proNGF-bands are observed, likely representing differently glycosylated proNGF-isoforms as reported by Reinshagen et al., 2000, Lobos et al., 2005, Pedraza et al., 2005 and Pundavela et al., 2014.

    Western Blotting Method: SDS-PAGE: denaturing and reducing, 10% Bis-Tris gel; Semi-Dry Transfer: Tris-Glycine (Towbins) buffer with 20% methanol; Membrane: PVDF (0.45 µm); Blocking: 5% skim milk in TBST, 1 hour at RT; Primary antibody: 5 µg/mL, overnight at 2-8°C; Secondary antibody: anti-rabbit-HRP (1/20,000), 2 hours at RT; Detection: Chemiluminiscence.