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Prominin-1, Rabbit Polyclonal Antibody
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Catalog Number
R-1824
Discontinued
- Product Name Prominin-1, Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Prominin-1 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded.
- Alternative Names Prominin-1, Prominin-like protein 1, PROM1, PROML1, Antigen AC133, MSTP061; CD133;
- Application(s) IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity This antibody is specific for CD133 as demonstrated by western blotting.
- Species Reactivity Human
- Immunogen Description E-coli derived human CD133/PROM1 recombinant protein (position: P531-H865) has been used as the immunogen. Human CD133 shares 61% amino acid sequence identity with mouse CD133.
- Conjugate Unconjugated
- Purity Description Affinity purified
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Prominin-1 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded.
- Application(s) IHC-Paraffin-embedded, WB
- Application Details Western Blot (0.1-0.5 µg/mL): tested in MCF-7 whole cell lysate. Immunohistochemistry: paraffin-embedded section (0.5-1.0 µg/mL) with HIER, pH 6.5; tested in human tonsil tissue. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Prominin-1
- Specificity This antibody is specific for CD133 as demonstrated by western blotting.
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description E-coli derived human CD133/PROM1 recombinant protein (position: P531-H865) has been used as the immunogen. Human CD133 shares 61% amino acid sequence identity with mouse CD133.
- Purity Description Affinity purified
- Format Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 200 µL sterile-filtered, ultrapure water to prepare 0.5 mg/mL solution. Centrifuge to remove any insoluble material.
- Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names Prominin-1, Prominin-like protein 1, PROM1, PROML1, Antigen AC133, MSTP061; CD133;
- Uniprot Number O43490
- Uniprot Number/Name O43490 (PROM1_HUMAN)
- Scientific Background May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). Ref: uniprot.org.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
