Alternative NamesBrain-derived neurotrophic factor (Precursor); Abrineurin
Application(s)IHC-Frozen, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityUsed in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antibody is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
Species ReactivityHuman, Mouse, Other Mammals (Predicted), Rat
Immunogen DescriptionA synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Application DetailsIHC, WB. 1-5 µg/mL is recommended for both applications, Flow Cytometry (2μg/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityUsed in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antibody is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Other Mammals (Predicted), Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Purity DescriptionAffinity purified
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 20 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesBrain-derived neurotrophic factor (Precursor); Abrineurin
Scientific BackgroundBDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
A: Specific staining of proBDNF expressed in human neuroblastoma SH-SY5Y cell line by Flow Cytometry using cat # R-176-20. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to proBDNF (0.5 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 dilution) with incubation for 20 minutes in dark at room temperature. Negative Control: Normal Rabbit IgG (black dashed). Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Pro-BDNF staining within the rat spinal cord dorsal horn. Normal cryostat sections of adult rat cord were stained with recombinant human anti-proBDNF (R-176-20) and detected with HRP labelled anti-rabbit antibody. Dilution 1:2000 in Human mouse and rat (Image courtesy of Dr Xin-fu Zhou).
Specific ReferencesLe Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607Application: Human, WB.
Macias M et al. (2007) Locomotor exercise alters expression of pro-brain-derived neurotrophic factor, brain-derived neurotrophic factor and its receptor TrkB in the spinal cord of adult rats. Eur J Neurosci. 25(8):2425-44Application: Rat