Application DetailsWestern Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human HSP90 (alpha isoform 1) has a predicted length of 732 amino acids and MW of 85 kDa. A concentration of 1.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetHeat Shock Protein 90 (HSP-90)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human (WB, IHC); rat (WB); mouse (IHC);
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a region (798-815 aa) from human Heat Shock Protein 90 (HSP90).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHSP 86; Renal carcinoma antigen NY-REN-38; HSP90AA1; HSP90A; HSPC1; HSPCA; HSP90AB1; HSP90B; HSPC2; HSPCB; Lipopolysaccharide-associated protein 2; LAP2; LPS-associated Protein 2;
Scientific BackgroundHeat shock protein 90 (HSP90) is a highly conserved and ubiquitously expressed molecular chaperone. HSP90 plays important roles in folding newly synthesized proteins and stabilizing and refolding denatured proteins after stress. Two forms of HSP90, alpha and beta, are encoded by seperate genes.