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Vascular endothelial growth factor (VEGF), Rabbit Polyclonal Antibody
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Catalog Number
R-1712
- Product Name Vascular endothelial growth factor (VEGF), Rabbit Polyclonal Antibody
- Product Description Rabbit anti-Vascular endothelial growth factor (VEGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Alternative Names VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF;
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
- Species Reactivity Human, Mouse (Predicted), Rat
- Immunogen Description A synthetic peptide corresponding to a sequence at the N-terminus of human VEGFA(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.
- Conjugate Unconjugated
- Purity Description Affinity purified on antigen column
- Regulatory Status For research use only.
Product Info
- Product Description Rabbit anti-Vascular endothelial growth factor (VEGF) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
- Application(s) IHC-Frozen, IHC-Paraffin-embedded, WB
- Application Details Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Vascular endothelial growth factor (isoform VEGF206) has a predicted length of 232 residues and MW of 27 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target Vascular endothelial growth factor (VEGF)
- Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
- Target Host Species Human
- Species Reactivity Human, Mouse (Predicted), Rat
- Antibody Host Rabbit
- Antibody Type Polyclonal
- Antibody Isotype IgG
- Conjugate Unconjugated
- Immunogen Description A synthetic peptide corresponding to a sequence at the N-terminus of human VEGFA(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.
- Purity Description Affinity purified on antigen column
- Format Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
- Storage Instructions At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF;
- Uniprot Number P15692
- Uniprot Number/Name P15692 (VEGFA_HUMAN)
- Scientific Background Vascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. At least 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF189, VEGF165 and VEGF121 are widely expressed whereas the isoforms VEGF206 and VEGF145 are uncommon. Isoforms VEGF165 and VEGF121 are freely secreted whereas isoform VEGF189 remains cell associated.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.