Product DescriptionRabbit anti-Myristoylated alanine rich C kinase substrate (MARCKS) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Application(s)ICC, WB
Application DetailsWestern Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:10,000 - 20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMyristoylated alanine rich C kinase substrate (MARCKS)
SpecificityThe specificity of this antibody has been confirmed by WB. Does not react with rodent protein.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionRecombinant full length MARCKS expressed and purified from E. coli
Purity DescriptionAffinity Purified
FormatLyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMyristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate 80 kDa protein;Marcks; Macs;
Scientific BackgroundMyristoylated alanine-rich C-kinase substrate (MARCKS) is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ref: SWISSPROT.
Image of mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS) R-1380-50 (red) and Chicken polyclonal antibody to MAP2 C-1382-50. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
Left: Analysis of MARCKS expression (red) in HeLa cells by Immunocytochemistry. MARCKS protein was detected with rabbit antibody to MARCKS at 1:1,000 dilution. Cells were co-stained with a mouse anti-beta tubulin antibody (green). Blue: DAPI nuclear stain. The MARCKS antibody recognizes protein localized in the plasma membrane and cytoplasm. Right: Western blot analysis of MARCKS expression (green) in various cell line lysates using rabbit anti-MARCKS antibody (1:1,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6. The blot was probed simultaneously with mouse antibody to GAPDH (M-1376-250, red, 1:5,000). The strong band at 80 kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells (lane 5), but no reactivity is seen on rodent cells (lanes 6 and 7). The GAPDH antibody used as a loading control reveals a single band at 37 kDa in all preparations.
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