Neurotrophin-4/5 (NT-4/5), Sheep Polyclonal Antibody

Product Info

Product Name Neurotrophin-4/5 (NT-4/5), Sheep Polyclonal Antibody
Product Description google Sheep anti-Neurotrophin-4/5 (NT-4/5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
Alternative Names Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4
Application(s) ELISA, IHC-Frozen, Neutralize, WB
Antibody Host Sheep
Antibody Type Polyclonal
Specificity Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
Species Reactivity Human, Mouse, Primate, Rat
Immunogen Description Recombinant human NT4
Conjugate Unconjugated
Purity Description Protein G purified IgG
Regulatory Status For research use only.

Specifications

Product Description Sheep anti-Neurotrophin-4/5 (NT-4/5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA, Neutralize.
Application(s) ELISA, IHC-Frozen, Neutralize, WB
Application Details IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 2-10 µg/mL for immunohistochemistry, ELISA and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Neurotrophin-4/5 (NT-4/5)
Specificity Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
Target Host Species Human
Species Reactivity Human, Mouse, Primate, Rat
Antibody Host Sheep
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description Recombinant human NT4
Purity Description Protein G purified IgG
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4
Uniprot Number P34130
Uniprot Number/Name P34130 (NTF4_HUMAN)
Scientific Background FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Citations & References

Specific References Beros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Application: Rat, Block/Inhibit.

Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis Application: Rat, Block/Inhibit.

Feron F et al. (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: Rat, IHC.
General References Lodouichi (2000) J. Neuroscie. 20(6): 2155
Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69