Alternative NamesSynapsin-1; Brain protein 4.1; Synapsin I; SYN1
Application(s)IHC-Paraffin-embedded, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThis antibody is specific for Synapsin I as demonstrated by western blotting.
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionA synthetic peptide corresponding to a sequence at the C-terminus of human Synapsin I (662-705aa KSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASL FSD), identical to the related mouse and rat sequences.
Application DetailsWestern Blot (0.1-0.5 µg/mL): tested in SHG-44 cell lysate (human malignant glioma cell line), mouse and rat brain lysates, and rat cell line. Immunohistochemistry (0.5-1.0 µg/mL): tested in formalin fixed, paraffin embedded human glioma tissue with HEIR. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetSynapsin-1 (SYN1)
SpecificityThis antibody is specific for Synapsin I as demonstrated by western blotting.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide corresponding to a sequence at the C-terminus of human Synapsin I (662-705aa KSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASL FSD), identical to the related mouse and rat sequences.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesSynapsin-1; Brain protein 4.1; Synapsin I; SYN1
Scientific BackgroundThe synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via exocytosis at any one time. Synapsins are present in invertebrates and vertebrates and are somewhat homologous across evaluated vertebrates. (Ref: Pfam.org)
Left: Western blot analysis of Synapsin I expression in rat brain extract (lane 1), mouse brain extract (lane 2), and SHG-44 whole cell lysate (lane 3). Synapsin I at 78 kD was detected using rabbit anti-Synapsin I antibody at 0.5 µg/mL. Right: Analysis of Synapsin I expression in paraffin-embedded sections of human glioma tissue by Immunohistochemistry. Primary antibody: rabbit antibody to Synapsin at 1 µg/mL. The immunohistochemical section was developed using the DAB method.