D tag, Mouse Monoclonal Antibody

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Catalog Number
M-1301

    Product Info

  • Product Name D tag, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-D tag Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Application(s) ICC, IHC-Frozen, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Mouse monoclonal anti D-tag antibody recognises over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
  • Species Reactivity Species Independent
  • Immunogen Description A synthetic peptide (DYKDDDDK) coupled to KLH.
  • Conjugate Unconjugated
  • Purity Description Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-D tag Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
  • Application(s) ICC, IHC-Frozen, WB
  • Application Details Western Blotting (WB), Immunostaining (IS) and Immunoprecipitation (IP). Suggested dilutions: WB at 1:100-1:1,000, IS and IP at 1:100-1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target D tag
  • Specificity Mouse monoclonal anti D-tag antibody recognises over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
  • Target Host Species Species Independent
  • Species Reactivity Species Independent
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG2b, lambda
  • Clone Name 1E6
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide (DYKDDDDK) coupled to KLH.
  • Sequence DYKDDDDK
  • Purity Description Purified
  • Format Liquid. PBS, pH 7.4 with 0.05% sodium azide. See vial label for concentration.
  • Storage Instructions Stable for 1 year at -20°C from the date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Scientific Background The D-tag system utilises a short hydrophilic peptide (DYKDDDDK) that is fused to either the N- or C-terminus of the protein of interest. It can be used in conjunction with other tags such as the 6X His tag.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot analysis of 293 cells transfected with D-tagged vectors and with differing concentrations of the anti-D tag M-1301-100. Lane 1 contains the highest concentration (0.5 mg/mL) and Lane 4 contains the lowest (0.1 µg/mL).

    Citations & References