Application DetailsWestern Blotting (WB), Immunocytochemistry (ICC) and Immunoprecipitation (IP). Suggested starting dilutions are as follows: WB at dilutions of 1:1000, IC and IP at dilutions of 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide.
Storage InstructionsStable for 1 year at -20°C unopened. After opening we recommend aliquoting and storing at -20°C for up to 6 months. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
Specific ReferencesDittmann K. et al (2010) 'Nuclear EGFR shuttling induced by ionizing radiation is regulated by phosphorylation at residue Thr654' FEBS Lett. 2010 Sep 24;584(18):3878-3884. Kolev M.V. et al (2010) 'Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration Pharmacogenomics J. 2010 Feb;10(1):12-9.
General ReferencesPotala S. and Verma R.S. (2010) 'Targeting head and neck squamous cell carcinoma using a novel fusion toxin-diphtheria toxin/HN-1.' Mol Biol Rep. 2010 Aug 1.