Histidine-tag (His), Mouse Monoclonal Antibody

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Catalog Number
M-1311

    Product Info

  • Product Name Histidine-tag (His), Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Histidine-tag (His) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity His-tagged fusion proteins
  • Species Reactivity Species Independent
  • Immunogen Description Synthetic peptide containing 6 Histidine residues.
  • Conjugate Unconjugated
  • Purity Description IgG
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Histidine-tag (His) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Application(s) ICC, WB
  • Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunoprecipitation (IP). Suggested starting dilutions are as follows: WB at dilutions of 1:1000, IC and IP at dilutions of 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Histidine-tag (His)
  • Specificity His-tagged fusion proteins
  • Target Host Species Species Independent
  • Species Reactivity Species Independent
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG2b
  • Clone Name HIS.H8
  • Conjugate Unconjugated
  • Immunogen Description Synthetic peptide containing 6 Histidine residues.
  • Purity Description IgG
  • Format Liquid. PBS, pH 7.4 with 0.05% sodium azide.
  • Storage Instructions Stable for 1 year at -20°C unopened. After opening we recommend aliquoting and storing at -20°C for up to 6 months. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Scientific Background The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

    Citations & References

  • Specific References Dittmann K. et al (2010) 'Nuclear EGFR shuttling induced by ionizing radiation is regulated by phosphorylation at residue Thr654' FEBS Lett. 2010 Sep 24;584(18):3878-3884.
    Kolev M.V. et al (2010) 'Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration Pharmacogenomics J. 2010 Feb;10(1):12-9.
  • General References Potala S. and Verma R.S. (2010) 'Targeting head and neck squamous cell carcinoma using a novel fusion toxin-diphtheria toxin/HN-1.' Mol Biol Rep. 2010 Aug 1.