Product DescriptionMouse anti-Myc proto-oncogene protein (Myc) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Application DetailsWestern Blotting (WB), Immunostaining (IS), Immunoprecipitation (IP). Suggested starting dilutions as follows: WB at 1:1,000, IS and IP at 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMyc proto-oncogene protein (Myc)
SpecificityAntibody detects over-expressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Target Host SpeciesHuman
Species ReactivitySpecies Independent
Immunogen DescriptionA synthetic peptide (EQKLISEEDL) coupled to KLH.
Purity DescriptionProtein A affinity chromatography from mouse ascites fluid
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide. See vial label for concentration.
Storage InstructionsStable for 1 year at -20°C from the date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe Myc tag contains the amino acids Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu (E-Q-K-L-I-S-E-E-D-L) corresponding to amino acids 410-419 of human Myc. This tag is widely used for monitoring expression of recombinant proteins in bacteria, insect and mammalian cells.