Product DescriptionMouse anti-Simian Virus Type 5 tag (SV5) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Application(s)ELISA, IHC-Frozen, WB
Application DetailsWestern Blotting (WB), Immunohistochemistry (IHC) and ELISA. Suggested dilutions for WB at 1:500 - 1:1000, ELISA at 1:5,000-10,000 and IHC at 1:50-1:100. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Purity DescriptionAntibody was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor. Protein A affinity chromatography.
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide
Storage InstructionsStable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe V5 epitope corresponds to a region from Simian Virus Type 5 (SV5).
Western Blot analysis of Mouse monoclonal anti-V5 tag M-1317-100 in untransfected 293 cells (Lane 1) and 293 cells transfected with lacZ-V5 tagged protein (Lane 2).