His-Tag, Rabbit Polyclonal Antibody

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Catalog Number
R-1312

    Product Info

  • Product Name His-Tag, Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-His-Tag Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
  • Application(s) ELISA, ICC, IHC-Frozen, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity His-tagged fusion proteins
  • Species Reactivity Species Independent
  • Immunogen Description The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.
  • Conjugate Unconjugated
  • Purity Description Affinity purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-His-Tag Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
  • Application(s) ELISA, ICC, IHC-Frozen, WB
  • Application Details ELISA, WB, IP, IF, IHC. Western Blotting at a suggested concentration of 1:500~1:1000. ELISA at a suggested concentration of 1: 2500. IP 5 µg/sample. IF 5 µg/mL. IHC 5 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target His-Tag
  • Specificity His-tagged fusion proteins
  • Target Host Species Species Independent
  • Species Reactivity Species Independent
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.
  • Purity Description Affinity purified
  • Format Liquid. PBS, pH 7.4 with 0.02% sodium azide.
  • Storage Instructions Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Scientific Background The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western Blot analysis of Rabbit polyclonal anti-HIS tag R-1312-100. Lane 1 contains control 293 cells. Lane 2 contains 293 cells transfected with His-tagged S6. Lane 3 contains 293 cells transfected with His-tagged CREB.

    Citations & References