Product DescriptiongoogleRabbit anti-Myc Tag Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Antibody HostRabbit
Antibody TypePolyclonal
SpecificityThis polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Product DescriptionRabbit anti-Myc Tag Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsELISA and Western Blotting (WB). Suggested dilutions for ELISA at 1:1,000 and WB 1:500 - 1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMyc Tag
SpecificityThis polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Target Host SpeciesHuman
Species ReactivitySpecies Independent
Antibody HostRabbit
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionMyc epitope tag peptide
Purity DescriptionAffinity purified
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.
Storage InstructionsMaintain at -20°C prior to opening for up to 1 year. The product is also stable at 2-8°C undiluted for several weeks. After opening store at -20°C in undiluted aliquots for up to six months. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundThe Myc tag contains the amino acids Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu (E-Q-K-L-I-S-E-E-D-L) corresponding to amino acids 410-419 of human Myc. This tag is widely used for monitoring expression of recombinant proteins in bacteria, insect and mammalian cells.
Specific ReferencesMartel, D. et al. (2017) Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit. BioMed Res. Int. 2017 Article ID 4635605. Application: WB