Application DetailsThis antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. Binding of this antivbody is not sensitive to calcium. This antibody recognises both native and denatured protein.
TargetDDDDK-tag
SpecificityThis antibody recognises the DYKDDDDK-tag, a widely used epitope tag.
Target Host SpeciesSpecies Independent
Species ReactivitySpecies Independent
Antibody HostRabbit
Antibody TypeRecombinant
Antibody IsotypeIgG, kappa
Clone NameM2.1
ConjugateUnconjugated
Immunogen Description
Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.
Purity DescriptionPurified antibody, via affinity chromatography
FormatLiquid. PBS with 0.02% Proclin 300.
Concentration1 mg/mL
Storage InstructionsStore at 2-8°C for up to 1 month. For longer storage, aliquot and store at -20_C. Avoid mulitiple freeze thaw cycles.
Batch NumberPlease see item label.
Expiration DateStore at 2-8⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C for up to 12 months.
Alternative NamesFLAG-tag; Equivalent to FLAG antibodies from Sigma; DYKDDDDK; DDDK; AspTyrLysAspAspAspAspLy
Scientific BackgroundThis chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. Binding of this antivbody is not sensitive to calcium. This antibody recognises both native and denatured protein.