Green Fluorescent Protein Tag (GFP), Chicken Polyclonal Antibody

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Catalog Number
C-2102

    Product Info

  • Product Name Green Fluorescent Protein Tag (GFP), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Alternative Names Green fluorescent protein, GFP
  • Application(s) ICC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Specific for GFP, does not cross-react with mCherry.
  • Species Reactivity Species Independent
  • Immunogen Description Recombinant AcGFP protein expressed in and purified from E. Coli.
  • Conjugate Unconjugated
  • Purity Description Affinity-purified using the immunogen.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
  • Related Products Green Fluorescent Protein Tag (GFP), Goat Polyclonal Antibody
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  • Application(s) ICC, WB
  • Application Details Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Product Validation Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells.
  • Target Green fluorescent protein (GFP)
  • Specificity Specific for GFP, does not cross-react with mCherry.
  • Target Host Species Jellyfish
  • Species Reactivity Species Independent
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Recombinant AcGFP protein expressed in and purified from E. Coli.
  • Immunogen Length Full-length recombinant protein.
  • Positive Control GFP-transfected HEK293 cells.
  • Negative Control Non-transfected HEK293 cells.
  • Purity Description Affinity-purified using the immunogen.
  • Physical State Solid.
  • Format Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose and 5 mM sodium azide as preservative.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Green fluorescent protein, GFP
  • Uniprot Number Q6YGZ0
  • Uniprot Number/Name Q6YGZ0 (Q6YGZ0_9CNID)
  • Cellular Localization Intracellular, cytosolic.
  • Scientific Background The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395 nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
    This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization. This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
  • Shipping Temperature 25°C (ambient)
  • Country of Manufacture Australia
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Analysis of GFP fusion protein-transfected HEK293 cells by Immunocytochemistry. GFP expression (green) was detected with chicken antibody to GFP (red, 1:1,000). Blue: DAPI nuclear stain. The chicken antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear yellow in color. Top: merged image; bottom panel: individual fluorescence channels. Right: Western blot analysis of GFP expression (green) in transfected HEK293 cells with chicken anti-GFP antibody (1:1,000). Lane 1: molecular weight marker; Lane 2: non-transfected control cells; Lane 3: GFP-transfected cells; Lane 4: mCherry-transfected cells. A strong band at ~27 kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein. The blot was simultaneously probed with a chicken antibody to HSP60 (red, lanes 2-4).

    Citations & References