Immunogen DescriptionGreen Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Application DetailsWestern Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Suggested starting dilution for WB of 1:5,000. For IHC-P, suggested dilution of 1:500 - 1:1000. Heat mediated antigen retrieval is recommended. For IHC-Fr, the suggested dilution is 1:1000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetGreen fluorescent protein (GFP)
SpecificityRecognizes GFP and its variants.
Target Host SpeciesJellyfish
Species ReactivitySpecies Independent
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionGreen Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Purity DescriptionIgY
FormatLiquid. PBS, pH 7.4 with 0.05% sodium azide. Concentration: 1mg/mL
Storage InstructionsStable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Adult mouse spinal cord sections. Neurons in the lamina 1 of the spinal cord were genetically engineered to express GFP under control of the prostatic alkaline phosphatase (PAP) promoter. Sections were fixed with 4% paraformaldehyde and paraffin embedded. Anti-Green Fluorescent Protein Tag (Chicken Polyclonal) was used at a dilution of 1:500.
Cholinergic neurons from the lumbar region of neonate mouse spinal cord, transfected with plasmid expressing GFP. Red fluorescence (B) shows ChAT immunoreactivity using rabbit anti-ChAT (R-043-100; 1:2,000). Green fluorescence (A) shows GFP expression using chicken anti-GFP (C-1322-100; 1:500) in these motor neurons.Scale bar: 50 µm. Courtesy of Kevin Smith, Flinders University
Brzezinski et al (2010) Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development. Development. 2010 Feb;137(4):619-29.
Stevens H.E. et al (2010) Fgfr2 is required for the development of the medial prefrontal cortex and its connections with limbic circuits. J Neurosci. 2010 Apr 21;30(16):5590-602.
Lattanzi A. et al (2010) Widespread enzymatic correction of CNS tissues by a single intracerebral injection of therapeutic lentiviral vector in leukodystrophy mouse models. Hum Mol Genet. 2010 Jun 1;19(11):2208-27.
Haegglund M. et al (2010) Activation of groups of excitatory neurons in the mammalian spinal cord or hindbrain evokes locomotion. Nat Neurosci. 2010 Feb;13(2):246-52.