Green Fluorescent Protein Tag (GFP), Chicken Polyclonal Antibody

Discontinued

Catalog Number
C-1322

    Product Info

  • Product Name Green Fluorescent Protein Tag (GFP), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC, IP.
  • Replacement Product

    Replaced by abc C-2102-100.

  • Alternative Names GFP;
  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Recognizes GFP and its variants.
  • Species Reactivity Species Independent
  • Immunogen Description Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
  • Conjugate Unconjugated
  • Purity Description IgY
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC, IP.
  • Replacement Product

    Replaced by abc C-2102-100.

  • Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB
  • Application Details Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Suggested starting dilution for WB of 1:5,000. For IHC-P, suggested dilution of 1:500 - 1:1000. Heat mediated antigen retrieval is recommended. For IHC-Fr, the suggested dilution is 1:1000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Green fluorescent protein (GFP)
  • Specificity Recognizes GFP and its variants.
  • Target Host Species Jellyfish
  • Species Reactivity Species Independent
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
  • Purity Description IgY
  • Format Liquid. PBS, pH 7.4 with 0.05% sodium azide. Concentration: 1mg/mL
  • Storage Instructions Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names GFP;
  • Uniprot Number P42212
  • Uniprot Number/Name P42212 (GFP_AEQVI)
  • Scientific Background GFP is a 27 kDa protein that emits a green fluorescence when excited by blue light.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Adult mouse spinal cord sections. Neurons in the lamina 1 of the spinal cord were genetically engineered to express GFP under control of the prostatic alkaline phosphatase (PAP) promoter. Sections were fixed with 4% paraformaldehyde and paraffin embedded. Anti-Green Fluorescent Protein Tag (Chicken Polyclonal) was used at a dilution of 1:500.

  • Cholinergic neurons from the lumbar region of neonate mouse spinal cord, transfected with plasmid expressing GFP. Red fluorescence (B) shows ChAT immunoreactivity using rabbit anti-ChAT (R-043-100; 1:2,000). Green fluorescence (A) shows GFP expression using chicken anti-GFP (C-1322-100; 1:500) in these motor neurons.Scale bar: 50 µm. Courtesy of Kevin Smith, Flinders University