SpecificityThis antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Species ReactivitySpecies Independent
Immunogen DescriptionPurified his tagged Fab fragment.
ConjugateUnconjugated
Concentration1 mg/mL
Purity DescriptionPurified antibody, via affinity chromatography
SpecificityThis antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Target Host SpeciesSpecies Independent
Species ReactivitySpecies Independent
Antibody HostMouse
Antibody TypeRecombinant
Antibody IsotypeIgG1, kappa
Clone Name3D5
ConjugateUnconjugated
Immunogen DescriptionPurified his tagged Fab fragment.
Purity DescriptionPurified antibody, via affinity chromatography
FormatLiquid. PBS with 0.02% Proclin 300.
Concentration1 mg/mL
Storage InstructionsStore at 2-8°C for up to 1 month. For longer storage, aliquot and store at -20_C. Avoid mulitiple freeze thaw cycles.
Batch NumberPlease see item label.
Expiration DateStore at 2-8⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C for up to 12 months.
Alternative Namespolyhistidine tag
Scientific BackgroundThis is a reformatted mouse IgG1 antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
M-2028-200 (1 µg/mL, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Primary incubation was 1 hour. Detected by chemiluminescence.