His-tag (C-term), Mouse Recombinant Antibody

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Catalog Number
M-2028

    Product Info

  • Product Name His-tag (C-term), Mouse Recombinant Antibody
  • Product Description google Mouse anti-His-tag (C-term) Recombinant Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names polyhistidine tag
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Recombinant
  • Specificity This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
  • Species Reactivity Species Independent
  • Immunogen Description Purified his tagged Fab fragment.
  • Conjugate Unconjugated
  • Concentration 1 mg/mL
  • Purity Description Purified antibody, via affinity chromatography
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-His-tag (C-term) Recombinant Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Target His-tag (C-term)
  • Specificity This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
  • Target Host Species Species Independent
  • Species Reactivity Species Independent
  • Antibody Host Mouse
  • Antibody Type Recombinant
  • Antibody Isotype IgG1, kappa
  • Clone Name 3D5
  • Conjugate Unconjugated
  • Immunogen Description Purified his tagged Fab fragment.
  • Purity Description Purified antibody, via affinity chromatography
  • Format Liquid. PBS with 0.02% Proclin 300.
  • Concentration 1 mg/mL
  • Storage Instructions Store at 2-8°C for up to 1 month. For longer storage, aliquot and store at -20_C. Avoid mulitiple freeze thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date Store at 2-8⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C for up to 12 months.
  • Alternative Names polyhistidine tag
  • Scientific Background This is a reformatted mouse IgG1 antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • M-2028-200 (1 µg/mL, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Primary incubation was 1 hour. Detected by chemiluminescence.

    Citations & References