Left: HEK293 cells transfected with a mCherry (red) were stained with chicken antibody to mCherry (green). Blue: DAPI nuclear stain. Transfected cells appear bright red, and co-stain for mCherry chicken antibody. Superimposition of both signals results in yellow-orange colour. Right: Western blot analysis of transfected and control HEK293 cell lysates, and recombinant proteins with chicken antibody to mCherry (1:2,000, green).  protein standard,  HEK293 control,  HEK293 cells transfected with mCherry,  recombinant mCherry protein,  recombinant GFP protein,  HEK293 transfected with GFP. The major band at about 30 kDa corresponds to mCherry protein and the slightly larger recombinant form runs at about 33 kDa due to presence of a His tag and other vector derived sequences. No cross-reactivity with GFP protein is observed. The same blot was simultaneously probed with a mouse antibody to HSP60 (red), which reveals a band at 60 kDa only in cell lysates.