Green fluorescent protein (GFP), Rabbit Polyclonal Antibody

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Catalog Number
R-1838

    Product Info

  • Product Name Green fluorescent protein (GFP), Rabbit Polyclonal Antibody
  • Product Description google Rabbit anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC.
  • Alternative Names Aequorea victoria green fluorescent protein
  • Application(s) IHC, WB
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Specificity GFP, cross reactivity with other mutant forms is expected as immunogen was a whole molecule GFP
  • Species Reactivity Species Independent
  • Immunogen Description Recombinant AcGFP purified from E. coli
  • Conjugate Unconjugated
  • Purity Description Affinity Purified
  • Regulatory Status For research use only.

    Specifications

  • Product Description Rabbit anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC.
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  • Application(s) IHC, WB
  • Application Details Immunocytochemistry (1:2,000 - 1:5,000) and Western Blot (1:1,000 - 1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Green fluorescent protein (GFP)
  • Specificity GFP, cross reactivity with other mutant forms is expected as immunogen was a whole molecule GFP
  • Target Host Species Jellyfish
  • Species Reactivity Species Independent
  • Antibody Host Rabbit
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description Recombinant AcGFP purified from E. coli
  • Purity Description Affinity Purified
  • Format Lyophilized from a solution containing PBS, pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide as preservative.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 uL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names Aequorea victoria green fluorescent protein
  • Uniprot Number Q6YGZ0
  • Uniprot Number/Name Q6YGZ0 (Q6YGZ0_9CNID)
  • Scientific Background The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (1). This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Left: Analysis of GFP expression (green) in transfected HEK293 cells by Immunocytochemistry. Cells were stained with rabbit anti-GFP antibody (red, 1:2,000). Blue: Hoechst staining for nuclei. Transfected cells appear orange-yellow due to the green (GFP) and red (anti-GFP antibody) color overlay. Right: Western blot analysis of GFP protein in transfected HEK293 lysate using rabbit antibody to GFP (green, 1:2,000). [1] protein standard, [2] non-transfected control cells, [3] transfected cells with GFP construct, [4] transfected cells with mCherry construct. A strong band at ~27 kDa corresponds to the expected molecular weight of GFP protein detected only in cells transfected with GFP construct. The antibody does not cross-react with mCherry protein. The same blot was simultaneously probed with a mouse antibody to beta-tubulin (red, ~ 50 kDa, lanes 2-4).

  • SDS Link

    SDS_R-1838-100_as_at_September2020.pdf

    Citations & References

  • General References Gurskaya NG et al. (2003) A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants. Biochem J. 373(Pt 2):403-