Western blot analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit antibody to mCherry (green, 1:1,000).  protein standard,  HEK293,  HEK293 cells transfected with mCherry-HA construct,  mCherry recombinant protein,  GFP recombinant protein, and  HEK293 transfected with a full length GFP construct. A major band at about 28 kDa corresponds to mCherry protein. The antibody does not cross-react with GFP protein. The same blot was simultaneously probed with a chicken antibody to HSP60 (red, lanes 2-6).