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ACRP30, Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2121
- Product Name ACRP30, Human, ELISA assay
- Product Description The human Survivin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Survivin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Survivin concentration are added to these wells and allowed to complex with the bound Survivin antibody. A biotinylated human Survivin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Survivin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Survivin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; API4; IAP4; Survivin;
- Application(s) ELISA
- Specificity Human Survivin
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, M1-D142
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 2 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Survivin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Survivin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Survivin concentration are added to these wells and allowed to complex with the bound Survivin antibody. A biotinylated human Survivin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Survivin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Survivin in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of ACRP30 in Culture Supernatant, Cell Lysates, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target ACRP30
- Specificity Human Survivin
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, M1-D142
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 2 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; API4; IAP4; Survivin;
- Uniprot Number O15392
- Uniprot Number/Name O15392 (BIRC5_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
