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Angiotensin-converting enzyme (ACE), Human, ELISA assay
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Catalog Number
BEK-2111
- Product Name Angiotensin-converting enzyme (ACE), Human, ELISA assay
- Product Description The human ACE Kit is a sandwich ELISA. The capture antibody is a monoclonal human ACE antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known ACE concentration are added to these wells and allowed to complex with the bound ACE antibody. A biotinylated goat anti-human ACE polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ACE present in the samples. The purpose of this kit is the in-vitro quantitative determination of human ACE in samples such as culture supernatant, serum, plasma (Heparin) and saliva. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
- Alternative Names Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; Kininase II; CD143; DCP; DCP1;
- Application(s) ELISA
- Specificity Human ACE
- Species Reactivity Human
- Immunogen Description Angiotensin-converting enzyme (ACE) converts angiotensin I to angiotensin II.
- Range 0.78ng/mL - 50ng/mL
- Sensitivity < 5 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human ACE Kit is a sandwich ELISA. The capture antibody is a monoclonal human ACE antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known ACE concentration are added to these wells and allowed to complex with the bound ACE antibody. A biotinylated goat anti-human ACE polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ACE present in the samples. The purpose of this kit is the in-vitro quantitative determination of human ACE in samples such as culture supernatant, serum, plasma (Heparin) and saliva. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Angiotensin-converting enzyme (ACE) in Culture Supernatant, Serum, Plasma (Heparin), Saliva. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Angiotensin-converting enzyme (ACE)
- Specificity Human ACE
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Angiotensin-converting enzyme (ACE) converts angiotensin I to angiotensin II.
- Range 0.78ng/mL - 50ng/mL
- Sensitivity < 5 pg/mL
- Sample Type Culture Supernatant, Plasma (Heparin), Saliva, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; Kininase II; CD143; DCP; DCP1;
- Uniprot Number P12821
- Uniprot Number/Name P12821 (ACE_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
