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Artemin, Human, ELISA assay
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US$547.00
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Catalog Number
BEK-2353
- Product Name Artemin, Human, ELISA assay
- Product Description The human Artemin kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human Artemin pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Artemin concentration are added to these wells and allowed to complex with the bound human Artemin antibody. A biotinylated polyclonal goat antibody to human Artemin is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human Artemin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Artemin in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Enovin; Neublastin; ARTN; EVN;
- Application(s) ELISA
- Specificity Human Artemin protein
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human Artemin expressed in an E.coli expression system; Immunogen sequence: A108-G220
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 2 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Artemin kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human Artemin pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Artemin concentration are added to these wells and allowed to complex with the bound human Artemin antibody. A biotinylated polyclonal goat antibody to human Artemin is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human Artemin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Artemin in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Artemin in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Artemin
- Specificity Human Artemin protein
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant human Artemin expressed in an E.coli expression system; Immunogen sequence: A108-G220
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 2 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), human Artemin protein standards, human Artemin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Enovin; Neublastin; ARTN; EVN;
- Uniprot Number Q5T4W7
- Uniprot Number/Name Q5T4W7 (ARTN_HUMAN)
- Scientific Background Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. It supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
