Product NameBone morphogenetic protein 2 (BMP2), Mouse, ELISA assay
Product DescriptiongoogleThe mouse Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BMP-2 in samples such as culture supernatant, serum and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesBMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;BMP-2;
Application(s)ELISA
SpecificityHuman; Mouse; Rat; The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
Product DescriptionThe mouse Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BMP-2 in samples such as culture supernatant, serum and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Bone morphogenetic protein 2 (BMP2) in Culture Supernatant, Tissue Homogenates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetBone morphogenetic protein 2 (BMP2)
SpecificityHuman; Mouse; Rat; The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
Target Cross-Reactivity (ELISA)There is no detectable cross-reactivity with other relevant proteins.
Target Host SpeciesMouse
Species ReactivityMouse
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesBMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;BMP-2;
Specific References1. Machado R et al (2012) Elastin-based nanoparticles for delivery of bone morphogenetic proteins. Methods Mol Biol. 2012;906:353-63.