Bone morphogenetic protein 2 (BMP2), Mouse, ELISA assay

As low as US$547.00
Only %1 left
Catalog Number
BEK-2006

    Product Info

  • Product Name Bone morphogenetic protein 2 (BMP2), Mouse, ELISA assay
  • Product Description google The mouse Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BMP-2 in samples such as culture supernatant, serum and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;BMP-2;
  • Application(s) ELISA
  • Specificity Human; Mouse; Rat; The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
  • Species Reactivity Mouse
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
  • Range 31.2 pg/mL - 2,000 pg/mL
  • Sensitivity < 2 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The mouse Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BMP-2 in samples such as culture supernatant, serum and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Bone morphogenetic protein 2 (BMP2) in Culture Supernatant, Tissue Homogenates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Bone morphogenetic protein 2 (BMP2)
  • Specificity Human; Mouse; Rat; The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Mouse
  • Species Reactivity Mouse
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
  • Range 31.2 pg/mL - 2,000 pg/mL
  • Sensitivity < 2 pg/mL
  • Sample Type Culture Supernatant, Serum, Tissue Homogenates
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;BMP-2;
  • Uniprot Number P21274
  • Uniprot Number/Name P21274 (BMP2_MOUSE)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References 1. Machado R et al (2012) Elastin-based nanoparticles for delivery of bone morphogenetic proteins. Methods Mol Biol. 2012;906:353-63.