Product NameBone morphogenetic protein 4 (BMP4), Human, ELISA assay
Product DescriptiongoogleThe human Bone morphogenetic protein 4 (BMP-4) Kit is a sandwich ELISA. The capture antibody is a monoclonal human BMP-4 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known BMP-4 concentration are added to these wells and allowed to complex with the bound BMP-4 antibody. A biotinylated human BMP-4 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BMP-4 in samples such as culture supernatant and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative NamesBMP4; bone morphogenetic protein 4; BMP2B; BMP-2B; BMP2B1; BMP-4;
Application(s)ELISA
SpecificityHuman BMP-4
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids S293-R408
Product DescriptionThe human Bone morphogenetic protein 4 (BMP-4) Kit is a sandwich ELISA. The capture antibody is a monoclonal human BMP-4 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known BMP-4 concentration are added to these wells and allowed to complex with the bound BMP-4 antibody. A biotinylated human BMP-4 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BMP-4 in samples such as culture supernatant and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s)ELISA
Application DetailsELISA. For the quantification of Bone morphogenetic protein 4 (BMP4) in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetBone morphogenetic protein 4 (BMP4)
SpecificityHuman BMP-4
Target Cross-Reactivity (ELISA)Mouse and rat leptin sequences show >80% amino acid identity with human leptin but direct cross reactivity to rodent or non-human species leptin's with this kit has yet to be tested.
Target Host SpeciesHuman
Species ReactivityHuman
Immunogen DescriptionThe standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids S293-R408
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage InstructionsStore at 2-8°C
Storage Temperature2-8°C
Batch NumberPlease see item label.
Expiration DateThis kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Alternative NamesBMP4; bone morphogenetic protein 4; BMP2B; BMP-2B; BMP2B1; BMP-4;